1. Yes, there is a difference. The 20th anniversary version is more arcade accurate. It also has some playable deleted scenes that never made it to the final arcade version. The graphics are supposed to be better, but to be honest, I never noticed a huge difference in quality.
2. There really is no major difference in DL2 and Space Ace, except, again, supposed to have better updated graphics. Space Ace is also more Arcade accurate.
3. The original DL DVD always shows Dirk coming back to life at the beginning of each scene. However, in the 20th anniversary, being more arcade accurate, this doesn't happen. And when Dirk dies, it will not make you repeat the scene over and over until you pass it. It will go to another random scene (again, this was more like the arcade) and eventually go back to the scene(s) you died at.
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